Table I.

Specificities of the generated anti-hCD154 mAbs

Anti-CD154 mAbsBinding to LTK-VectorBinding to LTK-CD154Binding to HEK 293-CD154Binding to Jurkat D1.1Binding to Jurkat E6.1- VectorBinding to Jurkat E6.1-CD154Binding to Activated T Cells
Control
5C8+++++++++++++++
Clone 3+++++++++++++++
Clone 4+++++++++++++++
Clone 5+++++++++++++++
Clone 6+++++++++++++++
Clone 7+++++++++++++++
Clone 8+++++++++++++++
Clone 10+++++++++++++++
Clone 11+++++++++++++++
Clone 13+++++++++++++++
Clone 14+++++++++++++++
  • Specificities of the generated mAbs in recognizing hCD154 expressed on the surface of different cell types are shown. LTK are mouse connective tissue fibroblasts (LTK-Vector are cells transfected with empty vector; LTK-CD154 are cells transfected with CD154). HEK 293 represents human embryonic kidney cells (HEK 293-CD154 are cells transfected with CD154). Jurkat E6.1 are human lymphoblastic T cells (Jurkat E6.1-Vector are cells transfected with empty vector; Jurkat E6.1-CD154 are cells transfected with CD154). Jurkat D1.1 is a subclone derived from Jurkat E6.1 constitutively expressing hCD154. These cells were stained for 30 min at 4°C with the various mAbs followed by 30 min at 4°C with GAM-Alexa-488, then analyzed by flow cytometry. The 5C8 mAb was taken as a control. Data presented are representative of five different experiments. Significant binding is represented by (+++) and no detectable binding is represented by (—).