Table II. CyTOF intracellular cytokine staining panel
SpecificityMetal TagSource
Dead cells115InIn-house
Beads140CeFluidigm
CXCR3139LaIn-house
CCR6141PrFluidigm
CD19142NdFluidigm
CD57143NdIn-house
CD69144NdIn-house
CD4145NdFluidigm
CD8146NdIn-house
CD3147SmIn-house
MIP1b148NdIn-house
CD85j149SmIn-house
CD45RA150NdIn-house
CD38151EuIn-house
TNF152SmFluidigm
Granzyme153EuIn-house
CD107a154SmIn-house
GMCSF155GdIn-house
CD154156GdIn-house
IL-2157GdIn-house
IFNg158GdIn-house
HLA-DR159TbIn-house
CD14160GdFluidigm
Ki67161DyIn-house
FOXP3162DyIn-house
ICOS163DyIn-house
IL-17164DyFluidigm
CD127165HoIn-house
IL-10166ErFluidigm
CD27167ErIn-house
CD33168ErFluidigm
CCR7169TmIn-house
PD1170ErIn-house
CXCR5171YbIn-house
IL-21172YbFluidigm
Perforin173YbIn-house
CD16174YbIn-house
CD56175LuIn-house
CD25176YbIn-house
DNA1191IrFluidigm
DNA2193IrFluidigm
  • Nonprotein subjects are shown in italics. 115In-Maleimide-DOTA, a live-dead stain, and 191Ir and 193Ir (DNA intercalators) are used to detect live intact singlets. The calibration beads allow us to normalize data for instrument performance.