Table III. HSV-1 protein recognition by antiserum from vaccinated mice
HSV-1 ProteinHigh Dose (1 × 105 PFU)Low Dose (1 × 103 PFU)Vehicle
UL39/ICP6/RR16,793,035 ± 1,303,967a352,954 ± 54,369372,251 ± 25,242
UL19/MCP/VP56,696,422 ± 890,026a1,843,337 ± 869,108535,912 ±151,606
UL29/ICP8/DBP5,597,621 ± 1,263,758a130,614 ± 90,58994,761 ± 50,909
UL27/gB4,453,171 ± 884,210a106,422 ± 63,34622,788 ± 11,578
UL22/gH2,223,914 ± 550,125a12,540 ± 12,5400 ± 0
UL18/TRX2/VP232,335,474 ± 487,355c967,491 ± 392,761d330,052 ± 60,015
UL40/RR21,773,914 ± 513,782a85,050 ± 24,62289,785 ± 21,998
UL44/gC1,329,966 ± 253,999a20,216 ± 99450 ± 0
US6/gD1,026,939 ± 191,329a0 ± 00 ± 0
UL38/TRX1/VP19c827,451 ± 263,020d219,067 ± 125,88128,706 ± 9146
US8/gE826,764 ± 169,833a72,489 ± 18,49541,676 ± 9301
UL35/SCP/VP26779,290 ± 127,433c239,978 ± 160,633d28,879 ± 22,879
UL1/gL554,211 ± 147,467a1386 ± 1386721 ± 721
UL48/VP16584,616 ± 145,499a99,682 ± 61,38232,467 ±15,242
UL31/NEC1584,149 ± 204,252b105,703 ± 28,17363,740 ± 23,269
UL26/VP24/21414,514 ± 125,223d130,212 ± 72,31941,565 ± 9047
UL12/NUC372,549 ± 87,858a65,219 ± 29,72883,159 ± 35,251
UL25/CVC2309,826 ± 75,562b104,840 ± 49,05051,411 ± 25,687
RS1/ICP4277,967 ± 73,595b67,645 ± 18,46329,843 ± 12,568
  • Serum from naive WT or HSV-1 0∆NLS–immunized mice (high and low dose) was used to immunoprecipitate viral-encoded proteins from HSV-1–infected Vero cells. Precipitated proteins were analyzed by MS. Proteins derived from HSV-1 were identified by cross-referencing derivative peptide ions with a reference sequence database. Numbers reflect matched peptide abundance/intensity per protein ± SEM by antiserum from HSV-1 0∆NLS– or PBS (vehicle)–vaccinated mice (n = 7 per group from three independent experiments).

  • a p < 0.01, comparing the high dose to the other groups of vaccinated mice.

  • b p < 0.05, comparing the high dose to the other groups of vaccinated mice.

  • c p < 0.01, comparing the indicated group to the vehicle group as determined by ANOVA and Scheffé multiple comparison test.

  • d p < 0.05, comparing the indicated group to the vehicle group as determined by ANOVA and Scheffé multiple comparison test.

  • WT, wild-type.