Table I. Primary Abs used in Western blot assay
AbSpeciesDilutionCatalog No.Brand
CD63 (H-193)Rabbit1:200SC-15363Santa Cruz Biotechnology (Dallas, TX)
CD 9 (EM-04)Rat1:500MA1-10309Thermo Fisher Scientific
Tsg101Rabbit1:1000ab30871Abcam (Cambridge, U.K.)
β-ActinMouse1:40004967SCell Signaling Technology (Danvers, MA)
GAPDHRabbit1:2000PA1-987Thermo Fisher Scientific
IκBαRabbit1:5009242SCell Signaling Technology
NF-κB (p105/p50)Rabbit1:1000ab32360Abcam
TBPMouse1:1000ab818Abcam
  • IκBα, inhibitor of NF-κB kinase subunit α, also known as IKK1; TBP, TATA-binding protein; Tsg101, tumor susceptibility gene 101 protein.