Table III. HX-MS analysis of cluster II–specific mAbs LE4, CH1, SWB1, 6C4, and WECH1
mAbClusterStrongly Protected Elementsa
PeptidesResiduesProximity
LE4II17–1963–69β-Strand d
67, 68154–161, 163α-Helices D and E
CH1II67, 68, 70, 71154–161, 163, 166, 167α-Helices D and E
SWB1II67–69154–161, 163α-Helices D and E
6C4II1964–68β-Strand d
83–88184–187, 189–199, 210, 203, 205–206α-Helices F and G
WECH1II2–414–20, 22–24α-Helix A
83–90185–187, 189–199, 201, 203, 205–207α-Helices F and G
  • a Actual exchange differences and entire RTA peptide list and corresponding residues are provided in Fig. 4 and Supplemental Table I.