Article Figures & Data
Figures
- FIGURE 1.
Diversity in Ly49H expression among CC006 mice.
(A–C) The frequency of (A) Ly49H NK cells among lymphocytes, (B) Ly49H NK cells among lymphocytes (excluding CC mice lacking Ly49H, ordered from least to greatest, and (C) Ly49H+ among NK cells (among those boxed in B, ordered from least to greatest) in the PBLs of B6, BALB/c, and CC mice. (D) Ly49H geometric mean fluorescence intensity (GMFI) by Ly49H+ NK cells (among those boxed in C, ordered from least to greatest). Data are from one to three individual experiments with 1–20 mice per group. Error bars represent SEM.
- FIGURE 2.
Selection of CC006 model for MCMV infection.
(A) Inheritance of Ly49H, MHC class I, MHC class II, MHC class Ib, and breeder information for CC strains identified in (Fig. 1D. (B) Frequency of Ly49H NK cells among lymphocytes for B6, BALB/c, CC006, and CC032 mice. (C and D) The number (C) and fold change (D) in the number (relative to prior to infection) of Ly49H NK cells per milliliter of blood at days 0, 3, 7, 15, and 30 after MCMV infection in B6 and CC006 mice. Data are representative of three independent experiments with 3–20 mice per group. Error bars represent SEM. *p < 0.05.
- FIGURE 3.
Generation and maintenance of adaptive NK cells in CC006 mice.
(A) Representative gating for NK cells, Ly49H staining among Ly49H NK cells, and gating of KLRG1 and CD62L subsets among Ly49H NK cells. (B–E) Frequency (B and D) and number per milliliter (C and E) of NK cells (B and C) and Ly49H NK cells (D and E) in naive and MCMV infected CC006 mice at days 0, 3, 7, 15, and 30 postinfection. (F and G) Ly49H GMFI (F) and frequency (G) of KLRG1+CD62L− cells among Ly49H NK cells in naive and MCMV infected CC006 mice at days 0, 3, 7, 15, and 30 postinfection. Data are representative of three independent experiments with five mice per group. Error bars represent SEM. *p < 0.05.
- FIGURE 4.
Generation of protective NK cell memory responses by immunization in CC006 mice.
(A) Experimental design. B6 and CC006 mice received i.v. transfer of either B6 splenocytes or B6-m157–expressing splenocytes, and no transfer mice served as an additional control. At day (D)3 and D5 posttransfer, mice that received splenocyte transfer were depleted of CD4 and CD8 T cells. Mice were bled at D7 to assess the impact of immunization on Ly49H NK cells. At D30 posttransfer, mice were infected with MCMV-Smith; a set of mice from each group were depleted of NK cells both 2 d prior to and on the day of infection. NK cell activation and viral titers were assessed at D33 posttransfer (D3 MCMV postinfection). (B–E) The number of Ly49H NK cells (B and C) and KLRG1+CD62L− Ly49H NK cells (D and E) in the spleen of CC006 (B and D) and B6 (C and E) mice 3 d after MCMV infection that were either naive, control immunized, or m157 immunized. (F) D3 liver MCMV titers in CC006 and B6 mice that were either naive, control immunized, or m157 immunized; infectious groups also included NK-depleted controls. Samples are representative of two independent experiments with four to eight mice per group. Error bars represent SEM. *p < 0.05.
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