Table II. Primers used in real-time PCR assays
GenesOrientationSequence
IL-6 F5′-3′5′-CCGGAGAGGAGACTTCACAG-3′
IL-6 R5′-3′5′-TCCACGATTTCCCAGAGAAC-3′
IL-1β F5′-3′5′-GGCAGGCAGTATCACTCATT-3′
IL-1β R5′-3′5′-AAGGTGCTCATGTCCTCATC-3′
TNF-α F5′-3′5′-GACGTGGAACTGGCAGAAGA-3′
TNF-α R5′-3′5′-GCCACAAGCAGGAATGAGAA-3′
TLR4 F5′-3′5′-ATATGCATGATCAACACCACAG-3′
TLR4 R5′-3′5′-TTTCCATTGCTGCCCTATAG-3′
GAPDH F5′-3′5′-CGAAGGTGGAAGAGTGGGAG-3′
GAPDH R5′-3′5′-TGAAGCAGGCATCTGAGGG-3′
rpL32A F5′-3′5′-GCTGGAGGTGCTGCTGATGT-3′
rpL32A R5′-3′5′-ACTCTGATGGCCAGCTGTGC-3′
  • F, forward primer; R, reverse primer; rpL32A, 60S ribosomal protein L32 A.