Table II. Ab conjugates used for the analysis in Fig. 2
TagTargetAb CloneSurface/IntracellularClusteringa
89YCD4530-F11SurfaceYes
141PrGr1 (Ly6C/Ly6G)RB6-8C5SurfaceYes
142NdCD11cN418SurfaceYes
143NdCD103-biotin/anti-biotin2E7 (BioLegend)/1D4C3 (anti-biotin)SurfaceYes
144NdMHC class I28-14-8SurfaceYes
145NdCD69H1.2F3SurfaceYes
146NdCD8α53-6.7SurfaceYes
150NdCD27LG.3A10SurfaceYes
151EuCD253C7SurfaceYes
152SmCD3ε145-2C11SurfaceYes (Fig. 2A)
No (Fig. 2B–D)
154SmCD11bM1/70SurfaceYes
156GdCD49b-PE/anti-PEHMa2 (BioLegend)/PE001 (anti-PE)Surface/secondaryYes
159TbPD-1 (CD279)RMP1-30SurfaceYes
160GdKLRG1-FITC/anti-FITC2FI (Thermo Fisher Scientific)/FIT-22 (anti-FITC)Surface/secondaryYes
161DyTbet4B10IntracellularYes
162DyLy6CHK1.4SurfaceYes
163DyLag3-allophycocyanin/anti-allophycocyaninC9B7W (BioLegend)/APC003 (anti-allophycocyanin)Surface/secondaryYes
164DyIkBαL35A5IntracellularYes
166ErCD196D5SurfaceYes
167ErCD150TC15-12F12.2SurfaceYes
168ErKi-67B56IntracellularYes
169TmIL10gfp5F12.4 (anti-GFP)IntracellularYes
170ErCD40LMR1SurfaceYes
171YbCD44IM7SurfaceYes
172YbCD4RM4-5SurfaceYes (Fig. 2A)
No (Fig. 2B–D)
173YbCD117 (ckit)2B8SurfaceYes
174YbMHC II (IA/IE)M5/114.15.2SurfaceYes (Fig. 2A)
No (Fig. 2B–D)
175LuCD127A7R34SurfaceYes
176YbICOS (CD278)7E.17G9SurfaceYes
195PtCisplatinCell-ID CisplatinNo
191Ir, 193IrIntercalatorCell-ID Intercalator-IrNo
140Ce 151Eu 153Eu 165Ho 175LuNormalization beadsNo
  • a Clustering parameters used for Fig. 2B–D excluded CD3ε, CD4, and MHC II.