Table I. Ab conjugates used for the analysis in Figs. 1 and 5
TagTargetAb CloneSurface/IntracellularClusteringa
89YCD4530-F11SurfaceYes
141PrGr1 (Ly6C/Ly6G)RB6-8C5SurfaceYes
142NdCD11cN418SurfaceYes
143NdGITR (CD357)DTA1SurfaceYes
144NdIL-2JES6-5H4IntracellularYes
145NdCD69H1.2F3SurfaceYes
146NdCD8α53-6.7SurfaceYes
148NdCD11bM1/70SurfaceYes
149SmCD196D5SurfaceYes
150NdCD253C7SurfaceYes
151EuCD64X54-5/7.1SurfaceYes
152SmCD3ε145-2C11SurfaceNo
153EuPD-L1 (CD274)10F.9G2SurfaceYes
154SmCTLA4 (CD152)UC10-4B9IntracellularYes
155GdIRF43E4IntracellularYes
156GdFoxp3-PE/anti-PEFJK-16s (Thermo Fisher Scientific)/PE001 (anti-PE)Intracellular/secondaryYes
158GdIL-10JES5-16E3IntracellularYes
159TbPD-1 (CD279)RMP1-30SurfaceYes
160GdGM-CSF–FITC/anti-FITCMP1-22E9 (Pharmingen)/FIT-22 (anti-FITC)Intracellular/secondaryYes
161DyTbet4B10IntracellularYes
162DyTNF-αMP6-XT22IntracellularNo (Fig. 1)
Yes (Fig. 5)
163DyLag3-allophycocyanin/anti-allophycocyaninC9B7W (BioLegend)/APC003 (anti-allophycocyanin)Surface/secondaryYes
164DyIκBαL35A5IntracellularYes
165HoIFN-γXMG1.2IntracellularNo (Fig. 1)
Yes (Fig. 5)
166ErIL-411B11IntracellularYes
167ErIL-6MP5-20F3IntracellularYes
168ErKi-67B56IntracellularYes
169TmLy-6A/E (Sca-1)D7SurfaceYes
170ErNK1.1 (CD161)PK136SurfaceYes
171YbCD44IM7SurfaceYes
172YbCD4RM4-5SurfaceNo
173YbCD117 (ckit)2B8SurfaceYes
174YbMHC II (IA/IE)M5/114.15.2SurfaceNo
175LuCD127A7R34SurfaceYes
176YbICOS (CD278)7E.17G9SurfaceYes
195PtCisplatinCell-ID CisplatinNo
191Ir, 193IrIntercalatorCell-ID Intercalator-IrNo
140Ce 151Eu 153Eu 165Ho 175LuNormalization beadsNo
  • a Clustering parameters used for Figs. 1 and 5 differed as indicated.