Table IV. Ab conjugates used for the analysis in the lung (Fig. 4A, 4C, 4E)
TagTargetAb CloneSurface/IntracellularClustering
89YCD4530-F11SurfaceNo
141PrGr-1 (Ly6C/Ly6G)RB6-8C5SurfaceYes
142NdCD11cN418SurfaceYes
143NdGITR (CD357)DTA1SurfaceYes
144NdMHC class I28-14-8SurfaceYes
145NdCD69H1.2F3SurfaceYes
146NdCD8α53-6.7SurfaceYes
148NdCD11bM1/70SurfaceYes
149Smp-4E-BP1 [T37/T46]236B4IntracellularYes
150NdCD253C7SurfaceYes
151EuCD64X54-5/7.1SurfaceYes
152SmCD3ε145-2C11SurfaceYes
153EuPD-L1 (CD274)10F.9G2SurfaceYes
154SmCTLA4 (CD152)UC10-4B9IntracellularYes
155GdIRF43E4IntracellularYes
156GdSiglec-F-PE/anti-PEE50-2440 (BD Pharmingen)/PE001 (anti-PE)Surface/secondaryYes
158GdFoxp3FJK-16sIntracellularYes
159TbPD-1 (CD279)RMP1-30SurfaceYes
160GdKLRG1-FITC/anti-FITC2FI (Thermo Fisher Scientific)/FIT-22 (anti-FITC)Surface/secondaryYes
161DyTbet4B10IntracellularYes
162DyTim3 (CD366)RMT3-23SurfaceYes
163DyLag3-allophycocyanin/anti-allophycocyaninC9B7W (BioLegend)/APC003 (anti-allophycocyanin)Surface/secondaryYes
164DyIkBαL35A5IntracellularYes
165Hoβ-catenin (active)D13A1IntracellularYes
166ErCD196D5SurfaceYes
167ErNKp4629A1.4SurfaceYes
168ErKi-67B56IntracellularYes
169TmLy-6A/E (Sca-1)D7SurfaceYes
170ErPD-L2-biotin/anti-biotinTY25 (BioLegend)/1D4-C5 (anti-biotin)Surface/secondaryYes
171YbCD44IM7SurfaceYes
172YbCD4RM4–5SurfaceYes
173YbCD117 (ckit)2B8SurfaceYes
174YbMHC II (IA/IE)M5/114.15.2SurfaceYes
175LuCD127A7R34SurfaceYes
176YbICOS7E.17G9SurfaceYes
195PtCisplatinCell-ID CisplatinNo
191Ir, 193IrIntercalatorCell-ID Intercalator-IrNo
140Ce 151Eu 153Eu 165Ho 175LuNormalization beadsNo