Table I. Abs and reagents
ReagentSourceCatalog Identifier
Abs
 Anti-CD16/32 (93)eBioscience14-0161-82
 Anti-B220 (RA3-6B2)–PerCP-eF710eBioscience47-0452-82
 Anti-FcεR1 (MAR-1)–PE-Cy7eBioscience25-5898-82
 Anti-NK1.1 (PK136)–PE, allophycocyanin-eF80eBioscience47-5941-82
47-5941-82
 Anti–PDCA-1 (eBio927)–allophycocyanin, FITCeBioscience17-3171-82
11-3172-82
 Anti-CD11c (N418)–AF700, allophycocyanineBioscience56-0114-82
17-0114-82
 Anti-CD11b (M1/70)–FITCeBioscience11-0118-42
 Anti-F4/80 (BM8)–allophycocyanin-eF780eBioscience47-4801-82
 Anti–MHC II (I-A/I-E M5/114.15.2)–eF450, PEeBioscience48-5321-82
12-5321-82
 Anti-CD8α (53-6.7)–allophycocyanineBioscience17-0081-82
 Anti-CD103 (2E7)–allophycocyanineBioscience17-1031-82
 Anti-CD80 (16-10A1)–PerCP-eF710, BiotineBioscience46-0809-42
13-0801-82
 Anti–PD-L1 (MIH5)–PerCP-eF710eBioscience46-5983-42
 Anti-CD86 (GL-1)–allophycocyanin, FITC, BiotineBioscience17-0862-82
11-0862-82
13-0862-82
 Anti–Ly-6C (HK1.4)–allophycocyanin-eF780, PE-Cy7eBioscience47-5932-82
25-5932-82
 Anti-CD19 (eBio1D3)–BiotineBioscience13-0193-82
 Mouse IgG2a (eBM2a)–BiotineBioscience13-4724-83
 Anti-CD3 (17A2)–BV605BioLegend100237
 Anti-CD3 (2C11)–BiotinThis articleN/A
 Anti-CD19 (6D5)–BV605, BiotinBioLegend115539
 Anti-B220 (RA3-6B2)–BV605BioLegend103243
 Anti-CD64 (X54-5/7.1)–BV711, allophycocyaninBioLegend139311
139306
 Anti-CD70 (FR70)–BiotinBioLegend104603
 Anti-OX40L (RM134L)–BiotinBioLegend108803
 Anti-GITRL (MIH44)–PEBD Biosciences563541
 Anti-Mouse 4-1BBL (19H3)–BiotinPurified from hybridoma provided by Dr. R. Miller, Emory University, and labeled using molecular probes N-hydroxy succinimidyl biotinN/A
 Anti-IFNAR1 Ab (MAR1-5A3), unconjugated/functional gradeBio X CellBE0241
 Anti–IFN-γRAb (GR-20), unconjugated/functional gradeBio X CellBE0029
 Mouse IgG1 isotype, unconjugatedBio X CellBE0083
 Rat IgG2a (2A3) isotype, unconjugatedBio X CellBE0089
Chemicals, peptides, and recombinant proteins
 Fixable viability dye eF506eBioscience65-0866-14
 Streptavidin-allophycocyanin (flow cytometry)eBioscience17-4317-82
 Streptavidin-PE (flow cytometry)eBioscience12-4317-87
 Murine IFN-βPBL Assay Science12405-1
 Murine IFN-γPeproTech315-05
 Brewer ThioglycollateSigma-AldrichB2551-500G
 BD Cytofix Fixation BufferBD Biosciences554655
 Collagenase IVInvitrogen17104019
 PercollGE healthcare17089101
  • N/A, not applicable.