Table I. Sequences of oligonucleotides
NumberConstructOrientationSequencea
2122GITRf5′-ATAGAAGCCCACCCTCGCTT-3′
2123GITRr5′-TGTTCTCAGTGCAGACTCGG-3′
2149GITR-Mycf5′-ATCTACGCGCAGGCCAAACATCATGCCCTAGTG-3′
2150GITR-Mycr5′-AATTGATCCCCAAGCTTCAGTGCAGACTCGGA-3′
2115GITRLf5′-ACTTGCCTCTGGCTGTCCTG-3′
2116GITRLr5′-GCACCAAGGACTCTTCCTTCCA-3′
2113GITRL-FLAGf5′-ACAGGTGCAGCTGCAGCTCCATAATGAACAGCC-3′
2114GITRL-FLAGr5′-GTGACACTATAGAATAGGAATTCTATATTTTATAAAGACCCCAG-3′
  • a Underlined sequences display overlapping sites with the vector.

  • f, forward; r, reverse.